Development of a method for the enantioselective analysis of methorphan and its O-desmethyl metabolite by liquid chromatography using tandem mass spectrometry

Author: Nino Gabekhadze
Keywords: Methorphan, Dexstromethorphan, Levomethorphan
Annotation:

The goal of my master's research was to come up with a way to separate methorphan and its enantiomers using high-performance liquid chromatography combined with mass spectrometry. To do this, we prepared a mixture of enantiomers of methorphan and its O-demethyl-metabolite as a sample. For the research, we used an Agilent Technologies 1290 Infinity liquid chromatograph along with the Agilent Technologies 6410 Triple Quad LC/MS. To carry out the experiment, we tried out 9 different mobile phase solutions and 9 chiral columns. The reason why enantioselective separation is important is because these substances have different pharmacological effects. In the end, the study revealed that the Lux Cellulose 3 column and MeOH + 0.1% H20 were the best choice for separating methorphan, its enantiomers, and metabolites. The method is highly efficient and rapid.

Lecture files:

მეთორფანის და მისი O-დემეთილ მეტაბოლიტის ენანტიოსელექტიური ანალიზის მეთოდის დამუშავება სითხური ქრომატოგრაფია ტანდემური მას სპექტრომეტრიის გამოყენებით [ka]