Detection of microbial indicators and potential pathogens in drinking and recreational water bodies in Tbilisi surroudings

Author: Mariam Alibegashvili
Keywords: Water quality, recreational waters, fecal indicators, phages, vibrios.
Annotation:

Water pollution is one of the most important problems facing the modern world, including Georgia. It can be assumed that climate change and global warming may cause serious genetic changes of clinically important microbes in water reservoirs of Georgia. The development of tourism and intensive migration in Georgia can be regarded as an additional contributing factor to the spread of non-endemic germs, including Vibrio Cholerae bacteria , in our water environment. That is why it is very important to regularly monitor water bodies in order to assess the current situation properly as well as reduce the risk of spreading any infection. The main target of the presented research is to determine the microbial indicators of pollution of the natural and artificial water bodies used for drinking and recreational purposes, Lake Lisi and the Tbilisi Sea, detection of potential pathogens, separation of primary isolates and their identification. Antibiotic profile of V. Cholerae isolates was studied using 15 antibiotics. High sensitivity was observed to gentamicin (92.6%), streptomycin (92.6%), sulfadiazine (88.9%), kanamycin (88.9%), chloramphenicol (88.9%) and ciprofloxacin (88.9%). 27 isolates of V. Cholerae were tested for susceptibility to bacteriophages. We used 11 phage clones specific to V. Cholerae O1 from different points of Georgia, isolated at different times and 2 standard phages from the Pasteur Institute collection. The results of the study showed that only 7 strains (25.9%) out of 27 were sensitive to phages isolated from the water of Lake Lisi. Based on phage susceptibility results, we can conclude that 20 phage-resistant V. cholerae strains სსdo not belong to the epidemiologically important V. cholerae serotype O1.

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მიკრობული ინდიკატორებისა და პოტენციური პათოგენების გამოვლენა თბილისის მიდამოებში არსებულ სასმელ წყალსა და რეკრეაციულ წყალსატევებში [ka]
მიკრობული ინდიკატორებისა და პოტენციური პათოგენების გამოვლენა თბილისის მიდამოებში არსებულ სასმელ წყალსა და რეკრეაციულ წყალსატევებში [ka]
მიკრობული ინდიკატორებისა და პოტენციური პათოგენების გამოვლენა თბილისის მიდამოებში არსებულ სასმელ წყალსა და რეკრეაციულ წყალსატევებში [ka]