Chiral stationary phases

In difference with totally porous silica core-shell silica consists of a solid core and porous shell. The advantage of core-shell silica for chromatographic separations relies on the fact that a surface area of such silica is commonly still sufficient for obtaining high performance separation while solid core does not allow analyte components to penetrate deep into the particles. This allows fast mass-transfer (fast transfer of the analyte between stationary and mobile phases) thus benefiting high plate numbers. Chiral stationary phases prepared based on core-shell type silica offer following advantages: smaller dead volume, higher plate numbers, negligible decrease of plate numbers with increasing separation speed (that allows fast separations without significant sacrifice of plate numbers). In addition, core-shell particles can be prepared with more uniform particle-size distribution that favors further increase of column efficiency. The major goal of the present project was to above mentioned the content of chiral selector in packing materials and chromatographic separation conditions will be optimized and their chromatographic study. The paper discusses: 1) Comparison of columns made with chiral selector supported on SPS and FPS particles; 2) Enantiomer resolving ability of SPS-based CSPs; 3) kinetic characterization - Plate number dependence on mobile- phase flow rate and instrumental requirements and chromatographic separation conditions will be optimized to fast separations.