The comparative analysis of the modern methods for determination of macrolides in honey

Author: giga akhalbedashvili
Keywords: macrolides, detection
Annotation:

Today, international health and nutrition organizations in the world are facing many challenges. Despite regulations and controls, there is still a danger of contamination of sake with pathogens, allergens, toxic compounds, pesticides, including antibiotic residues in honey - macrolides, which poses a threat to human health. Honey is a very useful product with its properties. In addition to organoleptic and pleasant aroma, it is used for medicinal purposes, in children's nutrition, medicine and others. Honey naturally has antiseptic, anti-inflammatory, antimicrobial properties. Due to many factors: environmental pollution, in veterinary medicine, agriculture, and beekeeping, macrolide antibiotics are used against diseases, which affect the quality of honey. The macrolide class of antibiotics also acts as an immunomodulator. Macrolides are also used to treat human diseases. Antibiotic resistance poses a significant threat to humanity. This problem is acute in Europe, America, developing and developed countries. As is known, food contaminated with antibiotic residues leads to the formation of resistance. This poses a potential threat to human health. Accordingly, the goal of our study was to validate, compare and test two different methods of macrolide antibiotics research. Methods that can reliably and accurately detect small residues and quantify the amount of contaminants and residues in honey. For the determination of macrolides, such parameters were selected that allowed us to improve the method and select significant and accurate ones. As a result, the mentioned method was validated according to the European Regulation 2002/657/EC, during which the selectivity, linearity, limit of quantification, repeatability, yield were determined - all satisfactory. We also conducted a test on samples of different types of honey, during which the yield of each macrolide was in the range of 80-120%, which meets the requirements of the European regulation.