”The study of CD180 expression and associated signalling pathways in the MEC 1 cell line.”

Author: Ilona Arutiniani
Keywords: CD180, CD150, MD1, MEC1
Annotation:

Annotation Chronic lymphocytic leukemia (CLL) is among the most common oncohematological diseases in Western countries, including Georgia. During CLL, proliferation and expansion of lymphocytes occur as a result of interaction with the microenvironment. One of the microenvironment recognition receptors is CD180 Toll-like receptor (TLR), which regulates the expansion and apoptosis of CLL cells. The presented work will begin to elucidate the complex immunological signalling which evidently underpins CLL. We will investigate whether MD1 expression patterns would affect CD180 expression and pro-apoptotic and pro-survival signaling mediated by CD180. The work will create new knowledge surrounding the functioning of both CD180 and CD150 which have been scarcely characterised in the context of cancer Our goal is to determine the significance of the expression and signaling pathways of the homolog, CD180, on co-receptor-mediated signal transduction in CLL cells. The goal is focused on optimizing the prognosis of the disease and predicting the prolongation of patients' lives. Within the framework of the work, we studied the expression of CD180, CD150, MD1 in the MEC1 cell line derived from CLL at different time points and in different phases of the cell cycle: we correlated CD180 between the expression of CD150, MD1 molecules and intracellular signaling pathways, the main signaling protein By measuring kinase phosphorylation in MEC1 cells. As a result of the study, it was established that MD1 is a positive regulator of the CD180 receptor in MEC1 cell culture; We determined the effect of surface and cytoplasmic MD1 on the expression of CD180 at different time points of the mentioned cell culture (correlation of sMD1 expression with sCD180 expression Corr.Cof=0.91, correlation of cytMD1 expression with sCD180 expression Corr.Cof=0.8). This suggests that the expression of certain other proteins may cause an increase in the expression of CD180. In Mec 1 cell cultures, the expression of CD150 was found to be high, at 48 hours, a decrease was observed compared to the 24 hour culture (MEC1 24სთ: 94.22±0.9%; 48 სთ 88.325±0.9%; 72 სთ: 99.515±0.26%, p=0.08). Which confirm that CD150 expression affects MD1 expression in the Mec1 cell line cells. As a result of the study, the importance of the expression of surface and cytoplasmic MD1 and co-stimulatory proteins in MEC1 cell culture was determined on the expression and function of CD180, the modulation of which is accompanied by apoptosis of leukemic cells. This, in turn, will create the basis for the prospects for the development of new approaches to CLL immunotherapy.