Separation of enantiomers of Clorpheniramine and Verapamil with neutral Cyclodextrins in Capillary Electrophoresis

Author: Mariam Shanidze
Co-authors: Ani Rurua
Annotation:

Capillary electrophoresis (CE) represents very useful method not only for separation of enantiomers of chiral drugs but also for better understanding of fine mechanisms of selector-selectand interactions. In the present study CE was used for separation of enantiomers of cationic chiral drugs, such as chlorpheniramine and verapamil with β-CD and its derivative, heptakis-(2,3,6-tri-O-methyl)-β-CD (TM-β-CD). Separation of enantiomers was performed in fused-silica capillary of 50 µm ID and 24 and 32.5 cm, effective and total lengths, respectively. The background electrolyte was 100 mM triethanolamine phosphate with pH=3.0. Various concentrations of cyclodextrins (CD) were used having in mind using CE for determination of selector-selectand association constants. The most interesting result of this study was that the enantiomers of all 4 analytes exhibited opposite affinity pattern towards studied β-CD and TM-β-CD.

Lecture files:

SEPARATION OF ENANTIOMERS OF CHLORPHENIRAMINE AND VERAPAMIL WITH NEUTRAL CYCLODEXTRINS IN CAPILLARY ELECTROPHORESIS [en]
ქლორფენირამინისა და დიმეთინდენის ენანრიომერების დაყოფა ნეიტრალური ციკლოდექსტრინებით კაპილარულ ელექტროფორეზში [ka]