Study of the decontamination potential of phage specific for methicillin-resistant staphylococcus isolated from the hospital environment in model trials

Author: Tamuna Kapanadze
Keywords: hospital-acquired staphylococcal infections, MRSA, Antibiotic sensitivity, phage sensitivity, decontamination
Annotation:

Methicillin-resistant (MRSA) staphylococcus is considered one of the main nosocomial pathogens and is an acute problem of modern medicine, especially in acute purulent-inflammatory disease occurring on the background of purulent wounds, trophic ulcers, diabetes. The treatment of hospital-acquired staphylococcal infections continues today with the use of antibiotics, but the inexorable increase in drug resistance has necessitated the use of other alternative biological agents, such as specific bacteriophages. The source of staphylococci isolated from two inpatient facilities in Tbilisi: Iashvili Central Children's Hospital and Thermal Injury Center (Khechinashvili Clinic) and Gori Military Hospital named after G. Abramishvili was the hospital environment (tracheostomes, monitor and bed surfaces). Therefore, the aim of the present study was to study the decontamination potential of MRSA specific bacteriophage in model trials. The following tasks were set: Study of antibiotic and phage sensitivity of staphylococcal clinical isolates Detection of the mecA gene determining resistance to methicillin in staphylococcal isolates. Study of the stability of staphylococcal phage lysis in the bare area. Determining the antibiotic resistance profile of MRSA staphylococcal strains isolated from clinical samples of different hospitals is very important for the epidemiological study of hospital infections and effective etiotropic treatment of patients. Virulence-specific bacteriophages active against MRSA may be used as an alternative to antibiotic therapy and also for decontamination of hospital environments, which will contribute significantly to the fight against infections caused by multidrug-resistant strains.