Assessment of the level of natural antibodies associated with thyroid-stimulating hormone in women with ovarian cyst

Background: Recently, a group of scientists the leadership of N. Porakishvili, he published work according to which 91.7% of women with ovarian cysts have a high level of naturally occurring IgG antibodies capable of binding to human chorionic gonadotropin (HCG) in their blood serum.ACG is a member of the family of glycoprotein hormones, and in addition to it, this family includes thyroid-stimulating hormone (TSH), follicle-stimulating hormone and malutheinizing hormone.Moreover, according to the literature, hypothyroidism, during which the level of TMH in the blood is increased, is one of the etiological factors of ovarian cyst development. Based on this, we became interested and set the goal of the study to evaluate whether the anti-Acg antibodies present in the blood of women with ovarian cysts are also related to TMH.Material and methods: peripheral blood sera of 41 women with ovarian cysts, 12 healthy volunteers, 6 patients with Hashimoto's thyroiditis and 3 patients with cystic inclusions in the thyroid gland were studied. We used an indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies capable of binding to TMH. In addition, in the same sera, we evaluated the TMH level using a sandwich ELISA.Results: Our results show that naturally occurring antibodies in the serum of women with ovarian cysts bind to TMH as well as to ACG. So, for example, the average optical density reflecting binding to TMH at 1: 50 dilution of sera is 0.629963 ± 0.036468, and to ACG is 0.516085 ± 0.049034. For comparison, in the group of control, healthy volunteers, the correlation index of serum of the same dilution with TMH is 0.177±0.028164 (p<0.0001), and with ACG is 0.073875±0.025576 (p<0.0001).For both antigens, a 50% titer was found at a 1:200 dilution of serum. A fairly high correlation was revealed between the titer indicators of antibodies capable of binding to the mentioned antigens. For example, for titer values of 50%, r=0.9319 In sera containing high levels of anti-THM antibodies (n=21), we also evaluated the level of the hormone itself, TMH. According to this part of the experiment, in 55% of women with ovarian cysts, the level of TMH in the blood serum is higher than in the blood serum of healthy volunteers.It should be noted that these women, along with the diagnosis of ovarian cyst, also have a diagnosis of hypothyroidism and undergo appropriate medical treatment. Conclusion: the detection of antibodies with the ability to bind to TMH in women with ovarian cysts puts on the agenda the need to continue research in several directions, the purpose of which will be to determine the cause of the appearance of these antibodies, to describe them in terms of epitope-specificity, subclass and affinity, and most importantly, to evaluate the impact These antibodies may have an effect on thyroid function.