„The evaluation of Ovarian cyst mucin specific antibodies binding to thyroid stimulating hormone“.

Author: Mariam sitchinava
Keywords: Ovarian cyst, mucin specific antibodies, TSH
Annotation:

Research prerequisite: Ovarian cysts are fluid-filled structures. Also, it is well known that ovarian cyst fluid is a rich source of certain types of mucins called “blood group antigens”. The formation of cysts can occur for many reasons. There is increasing evidence that ovarian cysts are often associated with an impaired thyroid function and hypothyroidism. A study in the Department of Immunology has shown that patients with ovarian cysts have natural antibodies that bind to human chorionic gonadotropin and thyroid-stimulating hormone. Due to the fact that these antibodies could not be detected in the blood of healthy volunteers, it was thought that they appeared in response to ovarian cyst mucins. On the basis of all this, the aim of the present study is to evaluate the ability of antibodies specific to ovarian cyst mucins to bind to thyroid-stimulating hormone (TSH). Research material and methods: The binding of the following commercial monoclonal antibodies to TSH and hCG was studied by immunoenzymatic analysis ELISA: ab212418 – monoclonal antibody against blood group antigen precursor (Abcam, USA); SPM522 – monoclonal antibody against blood group Lewis A antigen (Novus Biologicals, SPM522); ab3968 – monoclonal antibody against blood group Lewis B antigen (Abcam, ab3968); SPM297 – monoclonal antibody against mucin 5AC (Novus Biologicals, SPM297). In the next step, in sera isolated from the peripheral blood of 11 patients with ovarian cysts, we evaluated the inhibition of TSH binding to the hormone-specific antibody by commercial antibodies specific for ovarian cyst mucins. Results: The obtained results show that only one monoclonal antibody, ab212418, binds to TSH among the studied antibodies. Interestingly, the same antibody that binds to TSH was found to be able to bind to hCG. There is a high correlation between the binding rates of naturally occurring antibodies to these two hormones (r = 0.9967). It should be noted that the antibody ab212418 binds to the carbohydrate determinant - Gal1-3GalNAc-R. In the next stage of the study, we assessed whether the ab212418 antibody could inhibit the binding of TSH to the hormone-specific antibody. According to the obtained results, a trend of inhibition was observed in several samples, but statistically significant inhibition was found in only one sample. This can be considered natural because the research material contained an insufficient number of samples for statistical recording; however, the necessity of continuing the research is evident from the obtained results. Conclusion: In conclusion, it can be said that as a result of the presence of an ovarian cyst, antibodies appear in the body that can affect the level of thyroid-stimulating hormone.