Some technical aspects of enantioseparation using cellulose tris(3-chloro-5-methylphenylcarbamate) coated and covalently immobilized on silica in HPLC tandem MS

Author: Tamar Khatiashvili
Keywords: Enantiomers, Enantioselective, High-performance liquid chromatography-tandem mass spectrometry, Chiral selector
Annotation:

Since the number of chiral illicit drugs grows steadily the role of enantioselective analysis in forensic toxicology becomes increasingly important. Enantioselective high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) meets current requirements in this field more or less adequately and has the potential to assist in determination of cause of death. As our experience shows there are some technical issues the scientists have to consider in order to correctly perform enantioselective HPLC-MS/MS experiment and to interpret experimental results adequately. This research summarizes some of our observations on this regard. The enantiomers of over 100 chiral substances were separated on coated and covalently immobilized cellulose tris(3-chloro-5-methylphenylcarbamate) in HPLC and detected with serially connected UV-VIS and MS/MS detectors. The effect of a chemistry of a chiral selector, mobile phase composition, sample concentration on the peak shape, separation efficiency and ratio of response to both enantiomers was evaluated. It was found that mobile phase gradient (composition and especially the flow-rate), as well as sample concentration may drastically affect peak shape and ratio of response of MS detector to two enantiomers of a given chiral compound.

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